Composite

Part:BBa_M50021:Design

Designed by: Taylor Merkel, Chloe Thai, Julia Schulz   Group: Stanford BIOE44 - S11   (2016-10-27)


Heat-inducible d-Limonene synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1895
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1600
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1597
    Illegal SapI.rc site found at 319
    Illegal SapI.rc site found at 1572


Design Notes

All parts are optimized for expression in E. coli. Terminator was added to prevent expression of the GFP gene included in the plasmid this construct was going to be inserted into the E. coli promoter cassette plasmid provided by DNA 2.0.

Source

RBS and terminator are derived from bacteriophage T7; sequences provided by GeneDesigner software Promoter comes from groE gene in E. coli d-limonene synthase gene was sequenced from Citrus unshiu, then optimized for E. coli

References

GeneDesigner Wang & deHaseth, 2003 (promoter) Lindler, 1994 (promoter) Shimada, 2005 (d-limonene synthase)